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- W2367994910 abstract "Objective: To study the regulatory mechanism of PI3K signal pathway on osteoblast differentiation in pre-osteoblast MC3T3-E1. Methods:The activation of PI3K signal pathway was observed via the Western-blot experiment. When PI3K signal pathway was inhibited with the PI3K specific inhibitor LY294002, the change of osteoblast differentiation in MC3T3-E1 was observed based on the alkaline phosphatase assay and von Kossa staining. Results: The activation of PI3K signal pathway was observed along with osteoblast differentiation. When this pathway was blocked, osteoblast differentiation in preosteoblast MC3T3-E1 was significantly inhibited,which was justified by examining the ALP activity and bone nodule formation. Conclusion: PI3K signal pathway is activated along with the osteoblast differentiation, which is necessary for osteoblast differentiation in preosteoblast MC3T3-E1." @default.
- W2367994910 created "2016-06-24" @default.
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- W2367994910 date "2004-01-01" @default.
- W2367994910 modified "2023-09-25" @default.
- W2367994910 title "Blockage of PI3K signal pathway significantly inhibits osteoblast differentiation in pre-osteoblast MC3T3-E1" @default.
- W2367994910 hasPublicationYear "2004" @default.
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