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- W2367998396 abstract "Objective To investigate the effect of silencing her-2 gene by shRNA on the expressions of vascular endothelial growth factor(VEGF)-A and interleukin-8(IL-8) in human osteosarcoma saos-2 cells.Methods Recombinant expression vector with her-2 shRNA was constructed and transfected to saos-2 cells,using the cells untransfected as blank control and those transfected with plasmid shNC as negative control.The transcription levels of her-2,VEGF-A and IL-8 mRNAs in the transfected cells were determined by RT-PCR,while the expression levels of her-2 protein by Western blot.The secretion levels of VEGF-A and IL-8 in cell culture were determined by ELISA.Results The constructed Her-2 shRNA expression plasmid inhibited the expression of her-2 gene,of which the inhibiting rates to her-2 mRNA transcription and her-2 protein expression were 63.05% and 62.59% respectively.Both the mRNA transcription and protein expression levels of VEGF-A and IL-8 in saos-2 cells transfected with her-2 shRNA decreased significantly(P 0.01).Conclusion Both the mRNA transcription and protein expression levels of VEGF-A and IL-8 decreased significantly after her-2 gene silence,indicating that her-2 gene was involved in the regulation of VEGF-A and IL-8 expressions in osteosarcoma cells and might be used as a novel target for study on the molecular mechanism of angiogenesis of osteosarcoma." @default.
- W2367998396 created "2016-06-24" @default.
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- W2367998396 date "2012-01-01" @default.
- W2367998396 modified "2023-09-23" @default.
- W2367998396 title "Effect of silencing her-2 gene by shRNA on expressions of vascular endothelial growth factor-A and interleukin-8 in osteosarcoma cells" @default.
- W2367998396 hasPublicationYear "2012" @default.
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