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- W2368008881 abstract "Objective: To screen the antibodies of anti-HER2/neu receptor ligand domain 2(RLD2) from the phage library,and identify them by ELISA and MTT.Methods: The gene of RLD2 was amplified by PCR,and cloned into the pET-24a(+) expression vector and expressed in E.coli.The protein was purified and then renatured by dialysis.Four rounds of selection against RLD2 showed specific enrichment of phage antibody.The antibodies were identified by ELISA and MTT.Results and Conclusion: 6 clones which had higher appetency were obtained.Two clones of them were found that they could inhibit the growth of SKBR-3 breast tumor cells." @default.
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- W2368008881 date "2006-01-01" @default.
- W2368008881 modified "2023-09-28" @default.
- W2368008881 title "Screening and Identifying of Anti-RLD2 Antibodies from Phage Antibody Libraries" @default.
- W2368008881 hasPublicationYear "2006" @default.
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