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- W2368026658 abstract "Objective To construct a prokaryotic vector for expressing Helicobacter pylori adhesion A(hpa A) and cholerae toxin B(ctx B)fusion gene.Methods Amplify hpa A and ctx B genes by PCR and clone into expression vector pQE-30 to construct a recombinant plasmid pQE-hct,then transform to E.coli DH5α for expression of fusion protein HCT under induction of IPTG.Analyze the expressed product by Western blot and purify by nickel ion affinity column chromatography.Results The fusion gene fragment consisted of (1 161 bp) encoding 387 amino acid residues.SDS-PAGE showed that the expressed fusion protein, with a relative molecular weight of 40 000,contained more than 25% of total somatic protein.The purity of expressed protein after nickel ion affinity column chromatography reached more than 92%. Western blot showed specific recognition of expressed product with antisera against whole cell Hp and CT.Conclusion A prokaryotic vector for high expression of fusion protein HCT was successfully constructed.The study provided a basis for developing oral Hp vaccine." @default.
- W2368026658 created "2016-06-24" @default.
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- W2368026658 date "2004-01-01" @default.
- W2368026658 modified "2023-09-23" @default.
- W2368026658 title "Prokaryotic Expression of Helicobacter pylori Adhesion A (hpa A) and Cholerae Toxin B(ctx B)Fusion Gene" @default.
- W2368026658 hasPublicationYear "2004" @default.
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