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- W2368041696 abstract "Objectives:To establish an HPLC method to examine related substances in sulfasalazine.Methods:The column was Inertsil ODS column (250 mm×4.6 mm,5 μm).In a 1000 mL volumetric flask dissolve 1.13 g of sodium dihydrogen phosphate and 2.5 g of sodium acetate in 900 mL of water R.Adjust to pH 4.8 with glacial acetic acid R and adjust the volume to 1000 mL with water R,was used as the mobile phase A and Mix 1 volume of mobile phase A with 4 volumes of methanol methanol as mobile phase B with the flow rate of 1.0 mL·min-1,and gradient was 40%-100% in 60 min.The detection wavelength was 320 nm.Results:The linear range for sulfasalazine was excellent.Methods:At the same time,the HPLC method to examine salicylic acid and sulfapyridine was estabkished.with the same column,mobile phase at a flow rate of 1 mL·min-1 a mixture of 71volumes of mobile phase A and 29 volumes of mobile phase B with the pH 4.4,the detection wavelength was 300 nm.Results:The linear range for salicylic acid and sulfapyridine were excellent.The methods are simple,quick and reliable.Conclusion:It can be used to examine related substances and salicylic acid and sulfapyridine in sulfasalazine." @default.
- W2368041696 created "2016-06-24" @default.
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- W2368041696 date "2010-01-01" @default.
- W2368041696 modified "2023-09-23" @default.
- W2368041696 title "HPLC determination of related substances in sulfasalazine" @default.
- W2368041696 hasPublicationYear "2010" @default.
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