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- W2368049156 abstract "Objective To express and purify BL0033,BL0034 and their truncated mutants in fructose ABC transporter system of Bifidobacterium longum NCC2705,to verify the interaction between BL0033and BL0034,and to further determine the function region which mediates protein interaction.Methods Both bl0033 and bl0034 were cloned into vectors pGEX-4T-1 and pET32a and expressed by E.coli BL21.GST fusion protein and His fusion protein were purified by glutathione-Sepharose 4B beads and nickel column respectively.Interaction between BL0033 and BL0034 was analyzed by GST-pull down method.According to their function motif,truncated mutants of BL0033 and BL0034 were designed,cloned and expressed before GST pull-down assay was used to determine the interaction motif.Results The soluble fusion protein was expressed effectively in BL21 strain and purified by affinity chromatography.Obvious interaction was detected between BL0033 and BL0034.Furthermore,the functional area of BL0033 which could interact with BL0034 was the first motif 1-23 aa;truncated mutants 33-220 aa of BL0034 could strongly interact with BL0033 while the other two truncated mutants scarcely interacted with BL0033.Conclusion Strong interaction exists between BL0033 and BL0034,in which and motif 1-23 aa of BL0033 and motif 33-220 aa of BL0034 play an important role.This finding helps molecular mechanism studies on fructose ABC transporter of B.longum NCC2705." @default.
- W2368049156 created "2016-06-24" @default.
- W2368049156 creator A5030708251 @default.
- W2368049156 date "2010-01-01" @default.
- W2368049156 modified "2023-09-23" @default.
- W2368049156 title "Interaction analysis between BL0033 and BL0034 in Bifidobacterium longum" @default.
- W2368049156 hasPublicationYear "2010" @default.
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