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- W2368049238 abstract "Objective To evaluate the susceptibility of antifungal agents to Candida species with microdilution methods and compare the results of minimal inhibitory concentration(MIC) observed by visual and stereo-microscopic method.Methods According to CLSI M27-A3 microdilution method,the susceptibilities of 203 Candida isolates to antifungal agents: 4 kinds of azoles(miconazole,ketoconazole,econazole and bifonazole) and 4 kinds of non-azoles(nystatin,liranaftate,naftifine and terbinafine) were determined in vitro,and the results of MIC were observed by visual and stereo-microscopic method simultaneously.Results The agreements of MIC results of azoles observed by the 2 approaches were from 87.7% to 96.1%,and agreements of non-azoles were from 91.6% to 99.5%.Compared with the visual method,the stereo-microscope provided the following advantages: stereoscopic image,feasibility for observing the trace of fungi growth,easiness for distinguishing between the tested fungi and contamination strains.Except for liranaftate,naftifine and bifonazole,the other tested antifungals agents showed perfect fungistasis to Candida species in vitro.Conclusion Application of stereo-microscope should be advantageous for reading MIC results of susceptibility tested by microdilution method.The results of both the visual and stereo-microscope approaches presented fine agreement.Combination of the two methods is helpful for the estimation the MIC of antifungal agents in susceptibility research of antifungal agents in vitro." @default.
- W2368049238 created "2016-06-24" @default.
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- W2368049238 date "2011-01-01" @default.
- W2368049238 modified "2023-09-24" @default.
- W2368049238 title "Comparison of two approaches for observing susceptibility of antifungal agents to Candida species determined by microdilution method" @default.
- W2368049238 hasPublicationYear "2011" @default.
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