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- W2368049297 abstract "Objective:To investigate the colony formation capacity and P16 gene mRNA expression of high proliferative potential colony forming cells (HPP-CFC) derived from bone marrow hematopoietic cells of psoriatic patients,and probe into the relationship between the colony formation and P16 promoter mRNA expression. Methods:Bone marrow-derived mononuclear cells from the psoriatics and normal controls were purified by density gradient centrifugation. The bone marrow mononuclear cells were cultured in methylcellulose semi-solid culture medium with SCF,GM-CSF,IL-3 and IL-6 for 14 days. The colony cells were collected and the mRNA expression of P16 gene in colony cells was detected by using reverse transcription-polymerase chain reaction (RT-PCR). Results:In methylcellulose semi-solid culture system, the number and size of the colonies of HPP-CFC of psoriatic bone marrow were significantly less than those of the normal control with higher positive frequency of P16 gene mRNA expression compared with the normal control. Conclusion:The higher positive frequency of P16 gene mRNA expression in HPP-CFC may play a role in reducing HPP-CFC colony-forming capability of psoriatic hematopoietic cells." @default.
- W2368049297 created "2016-06-24" @default.
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- W2368049297 date "2007-01-01" @default.
- W2368049297 modified "2023-09-26" @default.
- W2368049297 title "Study on colony formation capacity and P16 gene mRNA expression of HPP-CFC derived from bone marrow hematopoietic cells of psoriatic patients" @default.
- W2368049297 hasPublicationYear "2007" @default.
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