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- W2368063964 abstract "Objective To develop an ELISA method for polyribosylribitolphosphate(PRP) of Haemophilus influenzae type b(Hib).Methods Rabbits were immunized with inactivated Hib,4 times a week for 6 weeks.Serum samples were collected,determined for antibody titer and subjected to immunoadsorption with non-specific antibody,from which rabbit anti-PRP antibody was purified.A double antibody sandwich ELISA method was developed by coating microtiter plate with the purified rabbit anti-PRP antibody,using HRP-labeled goat anti-rabbit IgG as the second antibody,then determined for optimal linear range of standard curve,and verified for specificity,accuracy and precision.Results The serum antibody titers of the immunized rabbits reached 1 ∶ 2 800.The linear detection range and minimum detection limit of the developed method were 0.030 ～ 0.500 ng / ml and 0.030 ng / ml respectively.All the detection results of protein in supernatant of E.coli,bovine serum albumin,LB medium and tetanus-Japanese encephalitis combined vaccine by the developed method were negative.The standard PRP at concentrations of 0.500,0.250 and 0.150 ng / ml were detected by the developed method,and the result showed that the intra-variation coefficient(CV) and recovery rate were 1.16% ～ 2.40% and 93.2% ～ 107.2%,while the inter-CV and recovery rate were 1.57% ～ 4.11% and 101.3% ～ 101.6%,respectively.Conclusion The developed ELISA method showed high specificity,accuracy and precision,which might be used for specific detection of PRP of Hib." @default.
- W2368063964 created "2016-06-24" @default.
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- W2368063964 date "2011-01-01" @default.
- W2368063964 modified "2023-09-25" @default.
- W2368063964 title "Development of an ELISA Method for Polyribosylribitolphosphate of Haemo-philus influenzae Type b" @default.
- W2368063964 hasPublicationYear "2011" @default.
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