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- W2368067575 abstract "Objective To count accurately the dendritic cells of lymph nodes by multiparameter flow cytometry.MethodsThe metastasis and non-metastasis nodes were obtained by endometrial carcinoma surgery,homogenizated to single-cell suspension with the concentration of 5×106/ml.Every case was divided into two tubes:one was added CD83 antibody only,the other was added both leukocyte common antigen CD45RO and CD83 antibody.The positive rates of CD83 antibody in both tubes were monitored by flow cytometry.ResultsCD83 expression was significantly different between metastasis group and non-metastasis group,and also different between double-labeling group and single-labeling group.ConclusionThe tumor metastasis is concerned with the decrease of the lymph node dendritic cells.The method labeled with leukocyte common antigen CD45RO and CD83 antibody could avoid the interference and enhance the accuracy.Meanwhile,it is necessary to count the very small dendritic cells by multiparameter flow cytometry." @default.
- W2368067575 created "2016-06-24" @default.
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- W2368067575 date "2007-01-01" @default.
- W2368067575 modified "2023-09-24" @default.
- W2368067575 title "Value of flow cytometry in monitoring lymph node dendritic cells" @default.
- W2368067575 hasPublicationYear "2007" @default.
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