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- W2368086066 abstract "Toxoplasma gondii GRA14 gene encoding dense granule protein 14 was amplified from 9 T.gondii strains from different geographical locations and hosts by PCR. The 9 PCR products were cloned into the pMD18-T vector and then transformed into Escherichia coli JM109,respectively.After sequencing,the relationships of 9 T.gondii strains were analyzed by bioinformatics software and a phylogenetic tree was constructed by Phylip 3.67. The B cell epitopes were predicted by DNAStar software.The length of the complete GRA14 sequences from the 9 T.gondii strains was 1227bp,and the A+T contents varied between 46.94% and 47.43%.GRA14 sequences were conserved among the T.gondii strains,and the variation compared with the ME49 strain was up to 0.73%.Phylogenetic analysis revealed that GRA14 gene sequence was not a suitable marker for studying genetic relationships of T.gondii isolates.However,the low variation among GRA14 gene sequences indicates it can be a vaccine candidate against toxoplasmosis. The B cell epitopes were most likely to form at 54-62aa,320-360aa,379-385aa and 400-408aa in GRA14 protein.The results of the present study have implications for further development of molecular vaccines against T.gondii infection." @default.
- W2368086066 created "2016-06-24" @default.
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- W2368086066 date "2013-01-01" @default.
- W2368086066 modified "2023-09-24" @default.
- W2368086066 title "Cloning and sequence analysis of GRA14 genes of Toxoplasma gondii isolates from different hosts and geographical locations" @default.
- W2368086066 hasPublicationYear "2013" @default.
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