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- W2368093013 abstract "PurposeThe aim is to prepare ouabain polyclonal antibody F(ab) 2 fragment and to estimate its molecular weight.MethodsOuabain polyclonal antibody was obtained from immunized rabbits.The antibody was digested with pepsin.The resulting products were analyzed and the molecular weight of F(ab) 2 fragment was estimated by HPSEC.The immune activity was detected by ELISA.Results100 mg of ouabain polyclonal antibody was digested by 2 mg of pepsin for 18 hours at pH 3.0 and active ouabain polyclonal antibody F(ab) 2 framgment was obtained.Its molecular weight was 107 kD.ConclusionThe active ouabain polyclonal antibody F(ab) 2 fragment could be prepared by digesting its antibody with pepsin." @default.
- W2368093013 created "2016-06-24" @default.
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- W2368093013 date "2001-01-01" @default.
- W2368093013 modified "2023-09-25" @default.
- W2368093013 title "Preparation and molecular weight estimation of ouabain polyclonal antibody F(ab)_2 fragment" @default.
- W2368093013 hasPublicationYear "2001" @default.
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