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- W2368095442 abstract "Objective: To study the association of lectin-like oxidized low density lipoprotein receptor-1(LOX-1) with angiotensin Ⅱ type 1 receptor (AT1) induced by oxidized low density lipoprotein(ox-LDL) using a bimolecular fluorescence complementation(BiFC) technique. Methods: LOX-1 and AT1 plasmids were transfected into COS7 cells. After treatment with ox-LDL(50ug/ml), cells were collected and proteins were extracted from them to be measured the expression level of p-ERK. The gene encoding AT1 or LOX-1 was cloned and then subcloned into bimolecular fluorescence complemented vectors.BiFC recombinant plasmids were obtained including phmKGN-MN- AT1, phmKGC-MN-AT as well as phmKGC-MC-LOX-1 and phmKGN-MC-LOX-1. The interaction between AT1 and LOX-1 was studied in HEK293 cells co-transfected with phmKGN-MN- AT1/phmKGC-MC-LOX-1 or phmKGC-MN-AT/phmKGN-MC-LOX-1 after stimulation with ox-LDL(50ug/ml). Other BiFC recombinant plasmids of β2-adrenergic receptor (β2AR), phmKGN-MN-β2AR and phmKGC-MN-β2AR, were also constructed and co-transfected with the corresponding BiFC recombinant plasmid of LOX-1, respectively. Fluorescence was observed after treatment with ox-LDL(50ug/ml). Results:The expression of p-ERK increased significantly in COS7 cells transfected with AT1 and LOX-1 plasmids after treatment with ox-LDL compared to control group. Plasmids of AT1, LOX-1, β2AR for bimolecular fluorescence complementation assay were successfully constructed. Green fluorescence was detected in HEK293 cells co-transfected with BiFC plasmids of AT1 and LOX-1 after stimulation with ox-LDL, while no green fluorescence was detected in HEK293 cells transfected with BiFC plasmids of β2AR and LOX-1. Conclusions: ox-LDL may induce the interaction between LOX-1 and AT1 receptor." @default.
- W2368095442 created "2016-06-24" @default.
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- W2368095442 date "2011-01-01" @default.
- W2368095442 modified "2023-09-27" @default.
- W2368095442 title "Investigation into the association of LOX-1 with AT1 receptor using BiFC." @default.
- W2368095442 hasPublicationYear "2011" @default.
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