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- W2368095927 abstract "Objective To construct single PCR assay for brucella,and optimize reaction conditions through simulative blood samples and inoculated mice,and provide laboratory evidence for PCR assay application in clinical diagnosis of brucellosis following-up. Methods Primer BP26 on genus level according to BP26 gene and three primers on strain level according to IS711 were designed; simulative samples of serial concentrations were constructed and mice were inoculated with M5 or Pps858-MCS2/M5 as experimental group,while PBS as control; brucella DNA was extracted by boiling method. This PCR assay was used to detect bacteria solution with primers BP26 and three strain primers,simulative samples and inoculated mice were diagnosed with primer BP26 and strain primer Meli. Results Designed primers on genus and strain level showed good specificity through brucella standard strains and vaccine strains; DNA was extracted from simulative samples and blood samples of the inoculated mice by the boiling method and specific bands were obtained; the PCR positive numbers of the inoculated mice increaseed early and desceased later,and increasing sample quantities increased the positive rate; the PCR positive rate of the boiling method was higher than chemical method at the same time point,and the PCR positive rate was more sensitive than traditional culture method. Conclusion The boiling method is convenient to extract DNA from blood. The PCR assay demonstrates higher sensitivity than traditional culture method; this PCR assay is very specific and available to be used for following-up of brucellosis patients." @default.
- W2368095927 created "2016-06-24" @default.
- W2368095927 creator A5088935942 @default.
- W2368095927 date "2009-01-01" @default.
- W2368095927 modified "2023-09-23" @default.
- W2368095927 title "Establishment of PCR assay for diagnosis of Brucellosis patients in following-up" @default.
- W2368095927 hasPublicationYear "2009" @default.
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