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- W2368102500 abstract "【Objective】The RNAi method was used to inhibit the expression of induced Hsp72 in the mouse embryonic fibroblast (MEF) to ascertain an effective siRNA of Hsp72. 【Method】Four pairs of siRNA specific for Hsp72 gene were designed for RNAi and were transferred into MEF through the help of LipofectamineTM 2000. The expression of Hsp72 mRNA and Hsp72 proteins was detected by RT-PCR and Western blot after heat shock of 41℃. 【Result】In the heat shock groups, the expression of Hsp70 mRNA and Hsp70 protein in the siRNA1, siRNA2 and the three control groups was significantly higher (P0.01) than that in the normal temperature group, and the expression of Hsp70 mRNA in siRNA3 and siRNA4 was significantly lower (P0.01) than that in the normal temperature group. The expression of Hsp70 protein in siRNA3 was significantly lower (P0.01) than that in the normal temperature group. While, there was no significant difference (P0.05) between siRNA4 and the normal temperature group. The siRNA3 and siRNA4 had a significant (P0.01) inhibiting effect on expression of Hsp70 mRNA in MEF compared with the blank control after heat shock. The inhibition rates were 86.2% and 75.4% (P0.01) for siRNA3 and siRNA4, respectively, the inhibition rates of siRNA3 and siRNA4 on Hsp72 protein expression were 77.3% and 57.0%, respectively (P0.01). 【Conclusion】 In the four siRNAs designed specific for Hsp72 gene, siRNA3 and siRNA4 target sites located on the surface of Hsp72 mRNA secondary structure can significantly reduce the expression of Hsp72 gene, but siRNA1 and siRNA2 target sites located on the interior of Hsp72 mRNA secondary structure had an insignificant inhibitory action on Hsp72 gene." @default.
- W2368102500 created "2016-06-24" @default.
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- W2368102500 date "2008-01-01" @default.
- W2368102500 modified "2023-09-26" @default.
- W2368102500 title "Ascertainment of Effective RNAi Sequences of Mouse Embryonic Induced Heat Shock Protein70" @default.
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