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- W2368130528 abstract "The paper reports the research about cloning and expression of an αglucosidase cDNA from Aspergillus niger in E.coli.cDNA was amplified by RT-PCR from total RNA of Aspergillus niger,and cloned into expression vector pSE380 which is controlled by Trc promoter,resultant pSE-αtg was transformed to E.coli BL21(DE_3).The positive transformants were fermented in flasks and induced by IPTG.The recombinant α-transglucosidase activity amounted to 485.40U/g,and showed the optimal activity at pH 6.0 and 45℃.The research also found that the recombinant enzyme was obviously activated by Cu~(2+) and Mn~(2+) ion.Adding maltose in the substrate can induce the production of α-glucosidase by E.coli transformant." @default.
- W2368130528 created "2016-06-24" @default.
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- W2368130528 date "2006-01-01" @default.
- W2368130528 modified "2023-09-23" @default.
- W2368130528 title "Cloning and Expression of An α-glucosidase cDNA from Aspergillus niger in E.coli" @default.
- W2368130528 hasPublicationYear "2006" @default.
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