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- W2368133661 abstract "Objective To isolate, culture and identify the human pancreatic stem cells in vitro and to observe the potency of the cells to differentiate into endocrine cells of pancreatic islet. Methods Stem cells were isolated from the human fetal pancreas by enzyme digestion for transfer of culture in vitro . The potency and types of the cell differentiation were then identified by using the pancreatic stem cell marker cytokeratin 19 (CK 19), the pancreatic β cell marker insulin and the pancreatic α cell marker glucagons with immunocytochemical staining. Results The shape of human pancreatic stem cells cultured in vitro were fusiform. The cells grew slowly in the first week but then proliferated quickly. The cells could be used for subculturing in vitro after 3 weeks. The subcultured cells could be used for subculturing again after another 2 weeks and the cells were found to be of CK 19 immunoreactive positive. After induced differentiation, the cells formed the pancreatic islet like structures. Part of the cells were of insulin immunoreactive positive but few of them were of glucagon immunoreactive positive. Conclusion Human pancreatic stem cells can proliferate quickly, may be of multipotency and can be differentiated into β and α cells of pancreatic islet in vitro." @default.
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- W2368133661 date "2003-01-01" @default.
- W2368133661 modified "2023-09-25" @default.
- W2368133661 title "Isolation, culture and identification of human pancreatic stem cells and their differentiation characteristics in vitro" @default.
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