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- W2368145429 abstract "AIM: To acquire GLUT4 cDNA from tissues of human muscles by RT PCR using special primers and to have it sequenced by cloning it into the vector of pGEM T. METHODS: GLUT4 cDNA segment was confirmed by BLAST 2.0 on NCBI, and using specific primers, the cDNA segment was acquired by RT PCR from tissue. Then it was cloned into the vector of pGEM 3zf (-) and sequenced automatically. RESULTS: The desired DNA was acquired by RT PCR using special primers from tissues of human muscles and the acquired cDNA fragment was cloned into vector of pGEM 3zf (-) and sequenced. The acquired DNA sequence was highly conservative. CONCLUTION: The entire GLUT4 cDNA can be acquired from tissues by RT PCR and the acquired DNA sequence is highly conservative in tissues from different people." @default.
- W2368145429 created "2016-06-24" @default.
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- W2368145429 date "2003-01-01" @default.
- W2368145429 modified "2023-09-23" @default.
- W2368145429 title "Cloning and sequence of GLUT4 cDNA from human muscles" @default.
- W2368145429 hasPublicationYear "2003" @default.
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