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- W2368162779 abstract "Objective To express hepatitis B virus(HBV)PreS2 fusion protein in Escherichia coli.The expression product was identified and purified.Methods The total HBV PreS2 protein gene was inserted into prokaryotic expression vector pMAL-C2x by DNA recombination technique.The recombinant plasmid was named pMAL-C2/S2 fusion vector.pMAL-C2/S2 was induced by IPTG in E.coli.The expression products were analyzed by SDS-PAGE.The fusion protein was purified by anion-exchange chromatography and Amylose Resin affinity chromatography.The furified protein was identified by Western blot and ELISA.The MBP tap protein of fusion protein was removed by Xa factor.Results The recombinant prokaryotic expression vector pMAL-C2/S2 was successfully constructed.The interest protein was HBV PreS2-MBP fusion protein and were expressed as inclusion body and soluble form.Western blot and ELISA results showed that fusion protein could react with anti-hepatitis B virus PreS2 antigen.The MBP tap protein of fusion protein was removed by Xa factor.Conclusion HBV PreS2 protein has been successfully expressed in E.coli and the resulted protein can be used in future as neotype preventive or immunotherapy reagent." @default.
- W2368162779 created "2016-06-24" @default.
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- W2368162779 date "2008-01-01" @default.
- W2368162779 modified "2023-09-25" @default.
- W2368162779 title "Expression of PreS2 gene from hepatitis B virus in Escherichia coli" @default.
- W2368162779 hasPublicationYear "2008" @default.
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