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- W2368168098 abstract "Objective: To establish an in vitro method to induce the differentiation of adult human bone marrow mesenchymal stem cells(BMSCs) into neural stem cells as the seed cells for the transplant therapy of neural system injuries.Methods: Human BMSCs were isolated from bone marrow and cultured for three passages(P3).The BMSCs were plated to the agarose-coated anti-adherent flasks,using the neural stem cell culturing medium including B27,EGF and bFGF to induce the differentiation of BMSCs to neurospheres.With the passage of neurospheres,neural stem cells(NSCs) proliferated.At last the NSCs markers in adherent cultured BMSCs and anti-adherent plus inducing cultured cells grew as neurospheres were compared with immunofluorescence and Western blotting.Results: Anti-adherent plus inducing culture can derive neurospheres from BMSCs.The primary neurospheres can be passaged to secondary neurospheres with proliferation.The cells in neurospheres expressed sonic hedgehog,neural cell adhesion molecule(NCAM),as well as the neural stem cell markers Nestin and CD133.Conclusion: The method combining anti-adherent with agarose and NSCs culturing medium can induce the differentiation of adult human BMSCs into neurospheres with NSCs characteristics,and is one promising strategy to provide large quantities of seed cells for repairing neural system injuries." @default.
- W2368168098 created "2016-06-24" @default.
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- W2368168098 date "2011-01-01" @default.
- W2368168098 modified "2023-09-24" @default.
- W2368168098 title "Induced differentiation of adult human BMSCs into neural stem cells" @default.
- W2368168098 hasPublicationYear "2011" @default.
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