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- W2368168671 abstract "Objective To construct a eukaryocyte expression vector of prohibitin 2gene,and establish the stable cell strain expressing the protein continuously.Methods PCR method was used to amplify the open reading frame of prohibitin 2from pLenti6/V5-D-TOPO-prohibitin 2-WT plasmid.Then the fragment was inserted into pcDNA3 vector after digested by KpnⅠand EcoRⅠto construct expression vector pcDNA3-prohibitin 2.SW620 cells were transfected with plasmid pcDNA3-prohibitin 2.Then the cells were selected under the pressure of G418 and Western blot was used to detect the expression of prohibitin 2 protein.Results Confirmed by sequencing,a eukaryotic expression vector encoding FLAG-prohibitin 2fusion protein was successfully constructed.FLAGprohibitin 2fusion protein could be expressed stably in SW620 cells after transfected with plasmid pcDNA3-prohibitin 2.Conclusion A stale cell strain over-expressing prohibitin 2 protein is successfully established." @default.
- W2368168671 created "2016-06-24" @default.
- W2368168671 creator A5040956172 @default.
- W2368168671 date "2015-01-01" @default.
- W2368168671 modified "2023-09-23" @default.
- W2368168671 title "Establishment of a stable SW620 cell strain expressing prohibitin 2 protein" @default.
- W2368168671 hasPublicationYear "2015" @default.
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