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- W2368171941 abstract "S1 genes of 12 strains of infectious bronchitis virus(IBV) isolated in China were amplified using RT-PCR, and the cDNA sequences of those strains were determined. Nucleotide sequence analysis was processedby comparingwith the published S1 genesequencesof other avian IBV strains in GenBank. Using the software of MEGA to analyze molecular phylogeny, a phylogenetic tree was constructed with neighbor-joining method and maximum parsimony method. The phylogenetic tree based on the nuc- leotide sequences of the S1 gene revealed that 12 Chinese IBV isolates had evolved intoone monogroupbut could be divided into twodistinct subgroups. The results showed that S1 gene of 11 IBV isolates was most closely related to that of other IBV strains isolated in China, while GX04-1 showed acloser phylogenetic relationship with European strain 4/91. The results suggested that there were a high variability among IBV isolates co-circulating in China and vaccines should be developed based on the local strains of IBV." @default.
- W2368171941 created "2016-06-24" @default.
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- W2368171941 date "2005-01-01" @default.
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- W2368171941 title "Sequence analysis of S1 gene of IBV strains isolated in China" @default.
- W2368171941 hasPublicationYear "2005" @default.
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