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- W2368172382 abstract "In order to detect the activity of anticolonic cancer scFv fragment, a primer containing E tag was designed for PCR amplification. The fusion gene scFv-E tag was cloned to plasmid pET-22b ( + )and was expressed in E. coli and the inclusion body of the main expression products was denatured and renatured. Activity analysis showed that the scFv fragment had the similar antigen affinity and specificity to the parent monoclonal antibody by using ELISA and immunochemi-cal methods." @default.
- W2368172382 created "2016-06-24" @default.
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- W2368172382 date "2003-01-01" @default.
- W2368172382 modified "2023-09-22" @default.
- W2368172382 title "Construction and Activity Analysis of Anticolonic Cancer scFv Fragment Containing E tag" @default.
- W2368172382 hasPublicationYear "2003" @default.
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