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- W2368181899 abstract "AIM To express nerve growth factor (NGF) of Naja naja atra(Chinese cobra) in E.coli by genetically engineered method and de- tect its biological activity.METHODS The cDNA of NGF was cloned into a secretive pro- karyotic expression vector pET-40b(+),which carries a C-terminal His Tag sequence.After transforming into E.coli BL21(DE3),NGF ex- pression was induced by isopropyl-thio-β-D-ga- lactoside(IPTG).The expressed product was purified by immobilized metal(Ni~(2+)) chelation affinity chromatography.The expressed NGF was testified by Western blot.The biological activi- ties of the recombinant NGF and natural NGF were measured by counting the number of PC12 cells with axon.RESULTS The antigen activ- ity of recombinant NGF was expressed by the presenting the 38 ku ladder in the Western blot. The rate of PC12 cells with axon was(83±3)% in natural NGF,and (21±3)% in the recombi- nant NGF.The results showed that the recombi- nant NGF has biological activity,but weaker than that of natural NGF.CONCLUSION The NGF with activity can be successfully expressed in E.coli." @default.
- W2368181899 created "2016-06-24" @default.
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- W2368181899 date "2007-01-01" @default.
- W2368181899 modified "2023-09-25" @default.
- W2368181899 title "Expression of nerve growth factor from Naja naja atra in E.coli" @default.
- W2368181899 hasPublicationYear "2007" @default.
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