Matches in SemOpenAlex for { <https://semopenalex.org/work/W2368183962> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2368183962 abstract "Objective To investigate the effects of lower concentrations of oxymatrine on culture-activation and transforming growth factor-β_1(TGF-β_1) stimulated activation of quiescent rat hepatic stcllatc cclls(HSCs), and the possible relations between the mechanism of the effects and TGF-β signaling via its receptors. Methods Freshly isolated HSCs from rat livers were subjected to treatment with lower concentrations of oxymatrine (0.25, 0.5, 1, 2, 4, 8 mg/L, respectively) at given period, and effects of oxymatrine on cell viability and proliferation were tested by MTT assay. Otherwise, HSCs were stimulated with TGF-β_1 (5 ng/mL), with or without oxymatrine (2 mg/L). Morphological features of HSCs were detected by microscopic observation, and TGF-β_1 mRNA, type Ⅰ and type ⅡTGF-β_1 receptor mRNA, Smad 3 mRNA and Smad 7 mRNA were analyzed by RT-PCR, while protein levels of α-SMA, Smad 2/3 and Smad 7 were evaluated by Western blot.Results Oxymatrine at the concentrations of 0.25-8 mg/L did not decrease the viability of primary cultured HSCs, while inhibited the proliferation of HSCs when reached a concentration of 8 mg/L. Oxymatrine (2 mg/L) prevented morphological transformation of HSCs stimulated by TGF-β_1 from the quiescent state to the activated one,and it also inhibited α-SMA expression stimulated by TGF-β_1.In parallel, RT-PCR showed an up-regulation of type Ⅰ TGF-β_1 receptor mRNA and Smad 7 mRNA in comparison with a decreased TGF-β_1 mRNA ( increased 1.25, 0.87 times or reduced 1.92 times than that of TGF-β_1 group, respectively, P0.05 ), while the expression of type Ⅱ TGF-β_1 receptor and Smad 3 mRNA was insignificantly altered by oxymatrine.The same changes of Smad 7 and Smad 2/3 protein were also conformed by Western blot. And similar effects were observed in HSCs activated by culture. Conclusion Lower concentration of oxymatrine (2 mg/L) has a significant inhibiting effect on culture-activation and TGF-β_1-stimulated activation of rat HSCs. It may execute its effects through the up-regulation of Smad 7, blockage of TGF-β_1 expression, increase of type Ⅰ TGF-β_1 receptor and restoration of the normal transmembrane signaling of TGF-β_1." @default.
- W2368183962 created "2016-06-24" @default.
- W2368183962 creator A5032600401 @default.
- W2368183962 date "2005-01-01" @default.
- W2368183962 modified "2023-09-28" @default.
- W2368183962 title "Effects of oxymatrine on hepatic stellate cells activation and its transmembrane signaling stimulated by TGF-β_1" @default.
- W2368183962 hasPublicationYear "2005" @default.
- W2368183962 type Work @default.
- W2368183962 sameAs 2368183962 @default.
- W2368183962 citedByCount "0" @default.
- W2368183962 crossrefType "journal-article" @default.
- W2368183962 hasAuthorship W2368183962A5032600401 @default.
- W2368183962 hasConcept C104317684 @default.
- W2368183962 hasConcept C105580179 @default.
- W2368183962 hasConcept C118131993 @default.
- W2368183962 hasConcept C126322002 @default.
- W2368183962 hasConcept C134018914 @default.
- W2368183962 hasConcept C1491633281 @default.
- W2368183962 hasConcept C153911025 @default.
- W2368183962 hasConcept C170493617 @default.
- W2368183962 hasConcept C176891718 @default.
- W2368183962 hasConcept C185592680 @default.
- W2368183962 hasConcept C2776245682 @default.
- W2368183962 hasConcept C2776415932 @default.
- W2368183962 hasConcept C2781080636 @default.
- W2368183962 hasConcept C53227056 @default.
- W2368183962 hasConcept C55493867 @default.
- W2368183962 hasConcept C62478195 @default.
- W2368183962 hasConcept C71924100 @default.
- W2368183962 hasConcept C86803240 @default.
- W2368183962 hasConcept C95444343 @default.
- W2368183962 hasConcept C98274493 @default.
- W2368183962 hasConceptScore W2368183962C104317684 @default.
- W2368183962 hasConceptScore W2368183962C105580179 @default.
- W2368183962 hasConceptScore W2368183962C118131993 @default.
- W2368183962 hasConceptScore W2368183962C126322002 @default.
- W2368183962 hasConceptScore W2368183962C134018914 @default.
- W2368183962 hasConceptScore W2368183962C1491633281 @default.
- W2368183962 hasConceptScore W2368183962C153911025 @default.
- W2368183962 hasConceptScore W2368183962C170493617 @default.
- W2368183962 hasConceptScore W2368183962C176891718 @default.
- W2368183962 hasConceptScore W2368183962C185592680 @default.
- W2368183962 hasConceptScore W2368183962C2776245682 @default.
- W2368183962 hasConceptScore W2368183962C2776415932 @default.
- W2368183962 hasConceptScore W2368183962C2781080636 @default.
- W2368183962 hasConceptScore W2368183962C53227056 @default.
- W2368183962 hasConceptScore W2368183962C55493867 @default.
- W2368183962 hasConceptScore W2368183962C62478195 @default.
- W2368183962 hasConceptScore W2368183962C71924100 @default.
- W2368183962 hasConceptScore W2368183962C86803240 @default.
- W2368183962 hasConceptScore W2368183962C95444343 @default.
- W2368183962 hasConceptScore W2368183962C98274493 @default.
- W2368183962 hasLocation W23681839621 @default.
- W2368183962 hasOpenAccess W2368183962 @default.
- W2368183962 hasPrimaryLocation W23681839621 @default.
- W2368183962 hasRelatedWork W1977874950 @default.
- W2368183962 hasRelatedWork W1990299002 @default.
- W2368183962 hasRelatedWork W2077326574 @default.
- W2368183962 hasRelatedWork W2134303113 @default.
- W2368183962 hasRelatedWork W2355203199 @default.
- W2368183962 hasRelatedWork W2356250659 @default.
- W2368183962 hasRelatedWork W2363497171 @default.
- W2368183962 hasRelatedWork W2365254064 @default.
- W2368183962 hasRelatedWork W2366949695 @default.
- W2368183962 hasRelatedWork W2370064173 @default.
- W2368183962 hasRelatedWork W2376223133 @default.
- W2368183962 hasRelatedWork W2382735060 @default.
- W2368183962 hasRelatedWork W2383660806 @default.
- W2368183962 hasRelatedWork W2393249508 @default.
- W2368183962 hasRelatedWork W2394205396 @default.
- W2368183962 hasRelatedWork W2401515453 @default.
- W2368183962 hasRelatedWork W2411758331 @default.
- W2368183962 hasRelatedWork W2413967248 @default.
- W2368183962 hasRelatedWork W3030818532 @default.
- W2368183962 hasRelatedWork W3030702612 @default.
- W2368183962 isParatext "false" @default.
- W2368183962 isRetracted "false" @default.
- W2368183962 magId "2368183962" @default.
- W2368183962 workType "article" @default.