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- W2368187777 abstract "Objective To construct replication-selective oncolytic adenovirus expressing lipocalin 2 gene and investigate its antitumor activity in vitro. Methods Lipocalin 2 gene was cloned into pCA13 plasmid to construct pCA13-lipocalin 2.PCA13-lipocalin 2 was digested with BglⅡ restriction enzyme to obtain the expression cassette including CMV promoter and lipocalin 2' CDS.This cassette was subcloned into pZD55 plasmid to produce pZD55-lipocalin 2.PZD55-lipocalin 2 and pBHGE3 plasmids were cotransfected and recombinated in 293 cells to obtain oncolytic adenovirus ZD55-lipocalin 2.After identifications for the novel oncolytic adenovirus by PCR and western blot,crystal violet staining and MTT were used to observe the inhibition of pancreatic cancer cell growth. Results The novel oncolytic adenovirus expressed lipocalin 2 proteins in PANC-1 cells;crystal violet staining and MTT showed that the oncolytic adenovirus markedly inhibited the growth of PANC-1. Conclusions The therapeutic oncolytic adenovirus expressing lipocalin 2 is successfully constructed,which would be a promising candidate for investigation of targeting gene-virotherapy of pancreatic cancer." @default.
- W2368187777 created "2016-06-24" @default.
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- W2368187777 date "2008-01-01" @default.
- W2368187777 modified "2023-09-22" @default.
- W2368187777 title "Oncolytic adenovirus expressing lipocalin 2 suppresses pancreatic tumor growth in vitro" @default.
- W2368187777 hasPublicationYear "2008" @default.
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