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- W2368191204 abstract "Objective:To study the effect of over expression of hypoxia inducible factor-1 alpha (HIF 1α) in esophageal epithelial Het-1A cells on the efficiency of photodynamic therapy (PDT). Methods:Stable over expression of HIF 1α in Het-1A cells was induced by incubation with cobalt chloride(CoCl_2). Cells were treated with photosensitizer 5-ALA followed by irradiation with visible red light at a wavelength above 600 nm. HIF 1α protein level was determined by western blot. The cell viability was tested by 3-(4,5-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) methods. siRNA was transfected into Het-1A cells by liposome mediation to knock down the HIF 1α expression. The apoptotic cell fraction was measured by TdT staining. Results:The over expression of HIF 1α can be induced by 4-h incubation with CoCl_2 100 μmol/L in Het-1A cells. Het-1A cells over expressed HIF 1α had a lower sensitivity to PDT than those without HIF 1α over expression. TdT staining showed that cell apoptotic rate decreased from 16% to 4% after incubation with CoCl_2. This could be further evidenced by the result that various concentrations of CoCl_2 could induce different levels of HIF1α protein, which was in accordance with the difference in cell viability after PDT. However, when the over expression of HIF 1α was knocked down by siRNA transfection, the sensitivity of the cells to PDT was recovered. Conclusion: Over expression of HIF 1α make Het-1A cells resistant to PDT. The attenuated PDT efficacy was achieved, at least in part, by inhibition of apoptosis. It suggested that decreasing the expression level of HIF 1α will help to improve the efficacy of PDT." @default.
- W2368191204 created "2016-06-24" @default.
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- W2368191204 date "2006-01-01" @default.
- W2368191204 modified "2023-09-25" @default.
- W2368191204 title "Inducible expression of HIF-1α in esophageal epithelia cells and its effect on photodynamic therapy" @default.
- W2368191204 hasPublicationYear "2006" @default.
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