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- W2368204659 abstract "The chromosomal number and karyotype in a marine shrimp Trachypenaeus curvirostris which is an important commereial species for the Chinese fisheries were studied using the materials of testicular tubules, embryos and nauplius larvae. The shrimp were collected from the coastal areas of Shilaoren, Qingdao, Shandong Province in April to July, 1990, 1994 and 1997.The tissue treament process is as follows.Embryos: They were collected in a beaker and immersed with colchicine solution. 1-1.5 hours later, the embryos were treated by hypotoniclysis firstly with seawater (50%) for 20min and then with KCl solution (0.075mol / L) for 10-20min.Another method is to undertake the hypotonic treatment with sodium citrate solution (0.3%) for 20-30min.Nauplius: They were placed in a beaker and immersed with colchicine solution. 2-2.5 hours later, the larvae were hypotonized with KCl solution for 20-30min.Adult shrimp: Colchicine was injected (1.5μg per body weight g) into the muscle of the first or the second abdominal segment, and kept alive in seawater for 4-6 hours. Individual shrimps were then sacrificed, the testis dissected out and cut into 2-3mm pieces. They were hypotonic with KCl solution(0.075mol / L) for 20-30min.All tissues were fixed with fresh Carnoy's solution (methanol: acetic asid = 3:1) three times.Chromosome preparation and observation: Embryo, larvae or pieces of testicular tubules were plased on a clean slide and spread using a forcep. The samples were stained with 5% Giemsa solution (pH = 7.0). The spread plates were observed and photographed with an Olympus microscope.Classification of chromosome was based upon the method of Levan et al (1964).The testicular tubules from the shrimp were used as the experimental materials for determining chromosome complements of meiotic bivalents. The somatic cells from the embryos and nauplius larvae were applied for both of karyotypes and the number of diploidy chromosom was determined.The results indicate that the chromosomal number of shrimp Trachypenaeus curvirostris is: n =35(2n = 70). According to Levan's standard, the chromosomal kareotype of Trachypenaeus curvirostris is 2n = 42M + 10SM + 12ST + 6T. No sex-chromosome in the complements was observed." @default.
- W2368204659 created "2016-06-24" @default.
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- W2368204659 date "1999-01-01" @default.
- W2368204659 modified "2023-09-24" @default.
- W2368204659 title "STUDY ON NUMBER AND KARYOTYPE OF A MARINE SHRIMP TRACHYPENAEUS CURVIROSTRIS" @default.
- W2368204659 hasPublicationYear "1999" @default.
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