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- W2368287718 abstract "A membrane-associated neopullulanase of Ruminobacter amylophilus 70 was extracted with 4% Triton X-100, and the amylase activity was obtained from the fraction of 70% ammonium sulfate pellet. After isoelectric focusing purification, and based on a SDS-PAGE detection, this membrane-associated neopullulanase was with a molecular weight of 91.2 kDa and a 5.8~5.9 pI. A different method of nondenatured enzymatic activity PAGE determined that the molecular structure of this membrane-associated neopullulanase protein was monomer, and the molecular weight of this membrane-associated neopullulanase was about 92.6 kDa. In Ruminobacter amylophilus 70 existed two kinds of amylases (soluble amylases and membrane-associated amylases such as neopullulanase) to work together for degradation of the starch molecule." @default.
- W2368287718 created "2016-06-24" @default.
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- W2368287718 date "2004-01-01" @default.
- W2368287718 modified "2023-09-25" @default.
- W2368287718 title "Purification and Analysis of Membrane-associated Neopullulanase of Ruminobacter amylophilus 70" @default.
- W2368287718 hasPublicationYear "2004" @default.
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