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- W2368290470 abstract "Objective:To investigate the molecular mechanism of operon comCDE regulating S.pn transformation.Methods:Thewild S.pn strains and their comE-defective derivations(△comE)were subjected to CSP-induced transformation.Transformation rates were calculated at different time points,and the mRNA expression levels of comE were detected by FQ PCR.The proteins of different expression among different strains were detected by two-dimensional electrophoresis and image analysis.Results:D39 could undergo transformation at 0、10 min and 20 min after CSP was added,and the transformation rate at 0min was the highest.After 10 minutes induction with CSP the mRNA level of comE was significantly higher than that of basal leve(lP0.05),and at 20minutes the mRNA level of comE was significantly decreased.For comE-defective strain,the transformation rate was 0.proteomics analysis displayed that six proteins were expressed significantly higher in △comE strains than that in D39 wild stains.The expression of six proteins increased significantly at 10 minutes after CSP was added,but reduced quickly at 20 minutes after CSP was added.The expression of two proteins were changed in both cases.The expression ofanother one protein was significantlydecreased after CSP was added.Conclusion:ComE is an essential gene for CSP-induced transformation,CSP probably induces some transformation related genes by comE-independent pathway.And comE probably has other fuctions than regulated-transformation,and it might be CSP-independent." @default.
- W2368290470 created "2016-06-24" @default.
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- W2368290470 date "2009-01-01" @default.
- W2368290470 modified "2023-09-25" @default.
- W2368290470 title "Investigation on the molecular mechanism of operon comCDE regulating the transformation of Streptococcus pneumoniae" @default.
- W2368290470 hasPublicationYear "2009" @default.
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