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- W2368291135 abstract "With the development of modern industry and agriculture, a major problem faced by the modern world:environmental pollution. The lack of affordable,effective approaches to environmental remediation has created a major need for development of novel approaches. Plant have many endogenous genetic, biochemical,and physiological properties that make them ideal agents for soil and water remediation. Significant progress has been made in recent years in developing native or genetically modified plants for the remediation of environment contaminants. Improvement of plants by genetic engineering open up new possibilities for phytoremediation. Tissue culture is prerequisite to plant genetic engineering. Alternanthera philoxeroides may be useful in remediation of environmental pollutants by transgenic engineering. But there is no report on tissue culture of Alternanthera philoxeroides. Alternanthera philoxeroides is dicotyledon. It was collected from the suburb of YangZhou in JiangSu Province. In this paper, it were studied that effect of NaClO、 AgNO 3 and HgCl 2 solution to sterilization of A. philoxeroides. Contamination rate were from 40% to 50% and germination rate were below 10% after stems with axillary bud of A. philoxeroides. were sterilized with 20% NaClO solution for 10—20 minutes. It was applicable to stems with axillary bud of A. philoxeroides to sterile with 1% AgNO 3 solution for 20—25 minutes. contamination rate of A. philoxeroides were from 11.1% to 0 and germination rate were from 55 6% to 50% or to sterile with 0.1% HgCl 2 solution for 3—5 minutes, contamination rate were from 25% to 0 and germination rate were from 58.3% to 44.4%.The results showed that the necessary time for killing bacteria contaminated in the tested material with 1% AgNO 3 was 20—25 minutes of with 0.1% HgCl 2 was 3—5 minutes. This study examined the effects of explants、media and exogenous phytohormone on tissue culture of Alternanthera philoxeroides . The stems,leaves and roots were used as the explants to study tissue cultue. Different basic media[MS、 1/2MS、 MS(1/2)、 B 5],0—0 5mg/L of indoleacetic acid(LAA),0—0 2mg/L of naphthyl acetic acid(NAA),0 5—5 0mg/L of 6-benzylaminopurine(6-BA) and 0 5—4.0mg/L of Zeatin (ZT)as additional compositions were used for inducing, differentiating and rooting tests.Induced results showed that 1/2 MS was more suitable for calli to grow and divide than B 5、MS(1/2)and MS. The stem and leaf of Alternanthera philoxeroides were suitable organs for tissue culture. Stemleaf was observed on growth rate. The combinations of NAA and BA or IAA and ZT can induce calli of the stem and leaf of Alternanthera philoxeroides , and the rate of calli induction increases with the increase of BA concentration. The higher rate of NAA/BA,the more roots from the calli format. It was thought that the results of this study could provide an useful basis for genetic transformation of Alternanthera philoxeroides ." @default.
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- W2368291135 date "2004-01-01" @default.
- W2368291135 modified "2023-09-28" @default.
- W2368291135 title "Callus induction and root differentiation from Alternanthera philoxeroides" @default.
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