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- W2368349642 abstract "Objective:To establish actinobacillus pleuropneumoniae Type 1 antiserum Actinobacillus actinomycetemcomitans(APP-1) lipopolysaccharide(LPS) monoclonal antibody hybridoma cell lines and to lay a foundation for diagnostic technique for typing.Methods:Intraperitoneal injection of formalin-fixed APP-1 strain vaccine antigen.Detected antibody titer of serum LPS by ELISA and chose the mouse with the highest value for cell fusion.Conducted and enhancing immunization three days before conventional methods of integration.Selectively cultivated fusion cells in HT and HAT medium.Cell culture supernatant was checked for LPS antibody using ELISA.Phsitive hybridoma cells were cloned using limited dilution.Results:2 strains secreting anti APP-1 antigen monoclonal antibody hybridoma cell lines of LPS were attained.Mouse ascites monoclonal antibody titer was 1:32 000-1:64 000.Conclusion:Three strains secreting anti-LPS APP-1 antigen monoclonal antibody hybridoma cell lines are successfully established,they produce monoclonal antibody without consensual reaction with the common pathogomic bacteria of respiratory passage and APP3,5,7,9." @default.
- W2368349642 created "2016-06-24" @default.
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- W2368349642 date "2007-01-01" @default.
- W2368349642 modified "2023-09-25" @default.
- W2368349642 title "Preparation and identification of the monoclonal antibodies of actinobacillus pleuropneumoniae in swine" @default.
- W2368349642 hasPublicationYear "2007" @default.
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