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- W2368364499 abstract "Objective: To investigate the inhibitory effect of recombinant adenovirus rAd-E1A-CDglyTK combined with enzyme prodrugs 5-flurocytosine(5-FC)and/or ganciclovir(GCV)on human epithelial ovarian carcinoma cell line SKOV3.Methods: The recombinant plasmid prAd-E1A-CDglyTK was transfected into 293 cells by liposome.After ampli- fication and purification,the virus was identified by PCR.The viral particle concentration was tested by ultraviolet spec- trophotometry and its infectious titer was tested through TCID50 assay,rAd-E1 A-CDglyTK was employed to infect SKOV3 cells for observation of the viral replication ability and the infection rate;the minimum MOI leading to above 90% infec- tion rate was determined.The drug sensitivity of the infected SKOV3 cells to 5-FC or GCV was observed by MTT assay and the IC_(50)values were determined.The growth inhibitory effects of rAd-E1A-CDglyTK or rAd-CDglyTK(at defined MOI)combined with 5-FC and GCV(at IC_(50))were determined on SKOV3 cells.Results: PCR results confirmed the presence of E1A gene and CDglyTK fragments in recombinant adenovirus.The viral particle concentration was 9.47×10~(11) VP/ml and its infectious titer was 3.16×10~(11)IU/ml,rAd-E1A-CDglyTK could replicate in SKOV3 cells;the infection rate of SKOV3 cells increased with the increase of MOI,the minimum MOI was 50 IU/cell.5-FC or GCV inhibited the growth of infected SKOV3 cells in a dose-dependent manner.The inhibitory effect of rAd-CDglyTK group([10.44±1.80]%)was significantly lower than that of rAd-E1A-CDglyTK group([16.57±1.59]%,P0.01).When combi- ning with 5-FC and GCV(at IC_(50)),the growth inhibitory rate reached(71.68±2.63)% in the tAd-E1A-CDglyTK/ 5-FC+GCV group,significant higher than that in the rAd-CDglyTK/5-FC+GCV([63.64±2.91]%,P0.01). Conclusion: Recombinant adenovirus rAd-E1A-CDglyTK combined with enzyme prodrugs can lead to a great cytotoxic effect on SKOV3 cells;the effect is superior to that of the replication-deficient adenovirus rAd-CDglyTK." @default.
- W2368364499 created "2016-06-24" @default.
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- W2368364499 date "2008-01-01" @default.
- W2368364499 modified "2023-09-25" @default.
- W2368364499 title "Inhibitory effect of recombinant adenovirus rAd-E1A-CDglyTK on human ovarian carcinoma SKOV3 cells" @default.
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