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- W2368367321 abstract "The optimal conditions for the synthesized culture medium of Clostridium perfringens A type is confirmed that the most suitable pH is about 8.0～8.5;the method of sterilisation in autoclave is 116 ℃,30 min;the used water is deionic water.For the strain(CVCC37) of Clostridium perfringens,the toxin activity attains 50～100 MLD/mL after growing in optimized medium for 6 hours,the toxic activity does not increase with even longer growth time;when compared with different culture temperature,the highest level of toxin activity at 36 ℃ or 37 ℃ is researched.Ultrafiltration with different membrane by MWCO(Molecular Weight Cutoff) is used to concentrate the culture supernatant,the harvest ratio of the 10 ku membrane by MWCO is 40%,and the discarded effluent results in 2/2 lethal ratio on rats injected by 0.2 mL in vein;while the harvest ratio of the 8 ku membrane by MWCO is 75% and the discarded effluent has no lethal activity;Therefore,the ultrafiltration with 8 ku membrane by MWCO is suitable for the ultrafiltration of cultured toxin produced by Clostridium perfringens A type." @default.
- W2368367321 created "2016-06-24" @default.
- W2368367321 creator A5067652261 @default.
- W2368367321 date "2012-01-01" @default.
- W2368367321 modified "2023-09-25" @default.
- W2368367321 title "The Research between the Application Parameter in the Synthesized Culture Medium of Clostridium perfringens A Type and the Ultrafiltration of Cultured Toxin" @default.
- W2368367321 hasPublicationYear "2012" @default.
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