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- W2368369541 abstract "A shoot-specific promoter was isolated in maize inbred line‘Zong 31'and was named as ZmSSP. By changing the CaMV35Spromoter in pCAMBIA3301into ZmSSP,a new plant expression vector pCAMBIA3301-ZmSSP-GUS was constructed,in which the reporter gene GUS was driven by ZmSSP.By Agrobacterium tumefaciens mediated method,transgenic tobacco lines were obtained,in which reporter gene GUSexpression patterns were analyzed.The results showed that ZmSSP had lower promoter activity than CaMV35S,and its pattern was quite different in different transgenic lines.Statistical data of 10transgenic lines showed that the highest GUS expression level in tobacco appeared in petiole(2.71times higher than that of Actin),and the following was in leaf and shoot.In root,very low GUS expression level was detected,which was 29.6percent of that of Actin,and 10.9percent of itself in petioles.The results indicate that ZmSSP is a good tissue-specific promoter in plant genetic engineering,suitable for trait improvement of underground vegetative organs." @default.
- W2368369541 created "2016-06-24" @default.
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- W2368369541 date "2014-01-01" @default.
- W2368369541 modified "2023-09-25" @default.
- W2368369541 title "Isolation of a Shoot-specific Promoter in Maize and Its Activity in Transgenic Tobacco" @default.
- W2368369541 hasPublicationYear "2014" @default.
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