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- W2368374462 abstract "Aim:To study the method of recombinant adeno-associated virus vector carrying human minidystrophin gene SMCKA3999 vector construct and expression in mdx mice (an animal model of DMD).Methods:We constructed recombinant adeno-associated virus vector carrying human minidystrophin gene SMCKA3999 vector (rAAVSMCKA3999) by three plasmids co-infection into 293 cells method and measured the titers of rAAVSMCKA3999 by dot blot hybridization. When injected into the skeletal muscle of mdx mice, gene expression was proved in mdx mice by Western BLot. Results:We can construct recombinant adeno-associated virus vector carrying human minidystrophin gene SMCKA3999 vector (rAAVSMCKA3999) by three plasmids co-infection into 293 cells method.The titers of rAAVSMCKA3999 will reach 5.0×10 10 .rAAVSMCK3999 resulted in efficient and stable expression persisting for 7 months. Conclusion:rAAVSMCKA 3999 we constructed has important significance for further gene therapy researches of DMD." @default.
- W2368374462 created "2016-06-24" @default.
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- W2368374462 date "2003-01-01" @default.
- W2368374462 modified "2023-09-24" @default.
- W2368374462 title "Recombinant Adeno-associated Virus Vector Carrying Human Minidystrophin Gene SMCKA3999 Vector Construction and Expression in mdx Mice" @default.
- W2368374462 hasPublicationYear "2003" @default.
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