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- W2368408889 abstract "The expression of Pfu DNA polymerase in recombinant E.coli BL21(DE3) induced by lactose was studied in this paper.We optimized the fermentation conditions,including initial biomass of induction,concentration of lactose and lasting time of induction.We also tried to purify Pfu DNA polymerase by JK110 weak acid cationic exchange column and Sephadex G-75 gel chromatography.The activity of purified Pfu DNA polymerase was assayed by PCR amplification.The result indicated that lactose could induce the expression of Pfu DNA polymerase effectively,and JK110 worked well in the purification.A total of 3 105 U of polymerase was obtained from 500 ml culture,and its specific activity was 22,200 U/mg.This result was better than that of IPTG." @default.
- W2368408889 created "2016-06-24" @default.
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- W2368408889 date "2008-01-01" @default.
- W2368408889 modified "2023-09-24" @default.
- W2368408889 title "Expression of Pfu DNA Polymerase Induced by Lactose and Its Purification" @default.
- W2368408889 hasPublicationYear "2008" @default.
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