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- W2368442017 abstract "The full-length DNA fragment of S gene of porcine transmissible gastroenteritis virus was amplified from the strain of TGEV JL by RT-PCR technique.Then,the fragment was connected to the pMD18-T vector. The SacⅠ and BamHⅠ restriction enzyme digestion analysis showed that the length of the product was 4 320 bp. After sequencing,S gene sequence of TH-98,etc. 8 TGEV strains were compared,and homology was 97.6%—99.8%. The gene was inserted into plant expression vector pBI121 to construct efficient plant expression vector at downstream of the CaMV35S promoter,inserted into Agrobacterium tumefaciens EHA101. The results showed that the recombinant plant expression vector pBI121-S and Agrobacterium tumefaciens strain were constructed." @default.
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- W2368442017 date "2010-01-01" @default.
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- W2368442017 title "Construction of plant expression vector of porcine transmissible gastroenteritis virus's gene." @default.
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