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- W2368447712 abstract "Objective:To establish a multiplex RT-PCR method for detecting 7 resistant marker genes expression simultaneously by using GeXP multi gene genetic analysis system.Method:Specific primers of 7 Candida albicans resistance marker genes,MDR1,FlU1,CDR1,CDR2,ADH1,ERG3,ERG11 and internal control gene,ITS were designed by GeXP eXpress Profile software.RNAs were isolated fromCandida tropicalis strains which were standard strain,strains with resistance,intermediate(dose dependent)and sensitive to FLuconazole using Yeast RNAiso Kit.The protocols for Gexp reactions were optimized and the results were analyzed by GeXP reaction software.Result:RNA expression of all 7 genes could be detected in the same reaction system.The relative expression levels of ERG11,CDR2,CDR1,MDR1 and ERG3 from Fluconazole resistance and dose dependent strains were higher than those of sensitive strains.Conclusion:A GeXP based multiplex PCR technique for detecting 7 Candida albicans resistance marker genes has been developed with advantages of higher throughput,stronger specificity and higher sensitivity." @default.
- W2368447712 created "2016-06-24" @default.
- W2368447712 creator A5023568331 @default.
- W2368447712 date "2014-01-01" @default.
- W2368447712 modified "2023-09-26" @default.
- W2368447712 title "Establishment of GeXP technique for detecting 7 resistant marker genes of Canadida spp" @default.
- W2368447712 hasPublicationYear "2014" @default.
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