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- W2368456944 abstract "Objective:To Construct and express pVAX-Hsp70-HSV2gD DNA Vaccine.Methods:Genes of Hsp70 and HSV-2gD were subcloned into vectors pVAX respectively.After confirmed by DNA sequence analysis,the recombinant plasmid pVAX-Hsp70-HSV2gD was transformed into COS-7 cells.The expressed protein was characterized by immunohistochemistry,SDS-PAGE and Western blot.Results:Recombinant plasmids were confirmed by DNA sequencing.The expressed protein analyzed by SDS-PAGE showed a new band with an apparent molecular mass corresponding to the predicted size(92 000).Immunohistochemistry and Western Blotting analysis demonstrated that gD protein was expressed in COS-7 cells.The level of γ-IFN in culture of pVAX-Hsp70-HSV2gD group was obviously higher than those of other groups(P0.05,respectively).Conclusion:The expression of the pVAX-Hsp70-HSV2gD DNA Vaccine was successful and laid a solid foundation for its further research." @default.
- W2368456944 created "2016-06-24" @default.
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- W2368456944 date "2007-01-01" @default.
- W2368456944 modified "2023-09-23" @default.
- W2368456944 title "Construction and expression of Hsp70-HSV2gD DNA vaccine" @default.
- W2368456944 hasPublicationYear "2007" @default.
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