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- W2368459717 abstract "The magnetic beads-based enzyme linked immunosorbent assay (mb-ELISA) was established for detection of organophosphorus pesticide.The magnetic bead-antibody complex applied in mb-ELISA was prepared by immobilization a general organophosphorus pesticides (OPs) antibody on the surface of magnetic beads.The optimization of mb-ELISA was found by means of uniform design with organic solvents,pH and the concentration of antibody used for coating magnetic beads as influence factors.The results showed that the interaction between organic solvents and the concentration of antibody had the greatest impact on IC50.Compared with the classical ELISA,the sensitivity of mb-ELISA was greatly enhanced in detecting OPs such as chlorpyrifos,quinalphos,trichlorfon,triazophos disulfoton,phosalone,parathion,dichlorvos and monocrotophos with the IC50 were decreased by 68.3%-95.6%." @default.
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- W2368459717 date "2010-01-01" @default.
- W2368459717 modified "2023-09-26" @default.
- W2368459717 title "Magnetic particle-based enzyme linked immunosorbent assay for detection of organophosphorus pesticide" @default.
- W2368459717 hasPublicationYear "2010" @default.
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