Matches in SemOpenAlex for { <https://semopenalex.org/work/W2368461010> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2368461010 abstract "AIM:To construct the eukaryotic expression vector(pEGFPN1-DNEGFR) carrying human dominant negative epidermal growth factor receptor(DNEGFR),detect the expression and the sub-cellular localization of dominant negative epidermal growth factor receptor-enhanced green fluorescence protein (DNEGFR-EGFP) in COS-7 cells transfected with pEGFPN1-DNEGFR. METHODS:The cDNA coding signal peptide,extracellular ligand-binding domain and membrane-spanning region of epidermal growth factor receptor(EGFR) precursor was obtained by reverse transcription-polymerase chain reaction(RT-PCR),then directionally cloned into the empty vector(pEGFP-N1) to construct pEGFPN1-DNEGFR.After confirmed by PCR amplification assay,double enzyme digestion,DNA sequencing and bioinformatics analysis of nucleotide sequence,pEGFPN1-DNEGFR was transfected into COS-7 cells,mediated by Lipofectamine 2000. The expression of and the sub-cellular localization of DNEGFR-EGFP in COS-7 cells were detected by Western Blot and Laser Scanning Spectral Confocal Microscope respectively. RESULTS:pEGFPN1-DNEGFR was constructed successfully,which was confirmed by PCR amplification assay,double enzyme digestion,DNA sequencing and bioinformatics analysis of nucleotide sequence. The expression of DNEGFR-EGFP was verified by Western Blot,and its predominant localization on the cell membrane of COS-7 cells was identified by Laser Scanning Spectral Confocal Microscope. CONCLUSION:The successful construction of pEGFPN1-DNEGFR,the expression and the sub-cellular localization of DNEGFR-EGFP in COS-7 cells,laid a solid foundation for further research on EGFR-targeted dominant negative strategy in cancer gene therapy." @default.
- W2368461010 created "2016-06-24" @default.
- W2368461010 creator A5013555971 @default.
- W2368461010 date "2009-01-01" @default.
- W2368461010 modified "2023-09-28" @default.
- W2368461010 title "Construction of the eukaryotic vector carrying human dominant negative epidermal growth factor receptor,its expression and sub-cellular localization in COS-7 cells" @default.
- W2368461010 hasPublicationYear "2009" @default.
- W2368461010 type Work @default.
- W2368461010 sameAs 2368461010 @default.
- W2368461010 citedByCount "0" @default.
- W2368461010 crossrefType "journal-article" @default.
- W2368461010 hasAuthorship W2368461010A5013555971 @default.
- W2368461010 hasConcept C104317684 @default.
- W2368461010 hasConcept C142613039 @default.
- W2368461010 hasConcept C153911025 @default.
- W2368461010 hasConcept C170493617 @default.
- W2368461010 hasConcept C187882448 @default.
- W2368461010 hasConcept C2776362946 @default.
- W2368461010 hasConcept C2779438470 @default.
- W2368461010 hasConcept C40767141 @default.
- W2368461010 hasConcept C49923114 @default.
- W2368461010 hasConcept C54009773 @default.
- W2368461010 hasConcept C55493867 @default.
- W2368461010 hasConcept C86803240 @default.
- W2368461010 hasConcept C92087593 @default.
- W2368461010 hasConceptScore W2368461010C104317684 @default.
- W2368461010 hasConceptScore W2368461010C142613039 @default.
- W2368461010 hasConceptScore W2368461010C153911025 @default.
- W2368461010 hasConceptScore W2368461010C170493617 @default.
- W2368461010 hasConceptScore W2368461010C187882448 @default.
- W2368461010 hasConceptScore W2368461010C2776362946 @default.
- W2368461010 hasConceptScore W2368461010C2779438470 @default.
- W2368461010 hasConceptScore W2368461010C40767141 @default.
- W2368461010 hasConceptScore W2368461010C49923114 @default.
- W2368461010 hasConceptScore W2368461010C54009773 @default.
- W2368461010 hasConceptScore W2368461010C55493867 @default.
- W2368461010 hasConceptScore W2368461010C86803240 @default.
- W2368461010 hasConceptScore W2368461010C92087593 @default.
- W2368461010 hasOpenAccess W2368461010 @default.
- W2368461010 hasRelatedWork W1895740739 @default.
- W2368461010 hasRelatedWork W1993155762 @default.
- W2368461010 hasRelatedWork W2009433981 @default.
- W2368461010 hasRelatedWork W2013546240 @default.
- W2368461010 hasRelatedWork W2023421684 @default.
- W2368461010 hasRelatedWork W2162885162 @default.
- W2368461010 hasRelatedWork W2324821870 @default.
- W2368461010 hasRelatedWork W2362208312 @default.
- W2368461010 hasRelatedWork W2363229546 @default.
- W2368461010 hasRelatedWork W2374702783 @default.
- W2368461010 hasRelatedWork W2379440382 @default.
- W2368461010 hasRelatedWork W2398776338 @default.
- W2368461010 hasRelatedWork W2403618778 @default.
- W2368461010 hasRelatedWork W2403719478 @default.
- W2368461010 hasRelatedWork W2410949163 @default.
- W2368461010 hasRelatedWork W2609093647 @default.
- W2368461010 hasRelatedWork W2616932098 @default.
- W2368461010 hasRelatedWork W2979139512 @default.
- W2368461010 hasRelatedWork W3147583008 @default.
- W2368461010 hasRelatedWork W2271192999 @default.
- W2368461010 isParatext "false" @default.
- W2368461010 isRetracted "false" @default.
- W2368461010 magId "2368461010" @default.
- W2368461010 workType "article" @default.