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- W2368483956 abstract "OBJECTIVE: To establish a HepG2 cell line with stable PXR silencing effect by a lentiviral vector carrying a short hairpin RNA(sh-RNA). METHODS:Three double-stranded sh-RNA targeting the PXR gene were designed,synthesized and cloned into the psi-sH1 sh-RNA vector. The effective recombinant sh-RNA expression plasmid against PXR,which was selected of HEK293T cells with three double-stranded sh-RNA plasmids,was packaged into HEK293T cells and used to infect HepG2 cells after 200 mmol/L puromycin screening for 2-3 weeks. The expression level of PXR was detected by western blot,the CYP3A4 mRNA levels both in sh-RNA PXR HepG2 stable cells and scramble control HepG2 cells were detected by real-time PCR. RESULTS:A recombinant sh-RNA expression plasmid against PXR gene with effective interference was successfully selected and a HepG2 cell line stably transfected with the sh-RNA PXR was established. CONCLUSION:A HepG2 cell line stably transfected with the sh-RNA P XR was established." @default.
- W2368483956 created "2016-06-24" @default.
- W2368483956 creator A5023432822 @default.
- W2368483956 date "2014-01-01" @default.
- W2368483956 modified "2023-09-23" @default.
- W2368483956 title "Establishment of a HepG2 cell line with stable PXR silencing by lentivirus-mediated RNA interference" @default.
- W2368483956 hasPublicationYear "2014" @default.
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