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- W2368500072 abstract "Based on the principle that tropina make animal body produce antibody,an indirect ELISA for detection of the antibody against bovine Clostridium perfringens type D was developed with the tropina from C.perfringens.In the indirect ELISA,the optimal concentration of the antigen from C.perfringens was 50 μg/mL,the optimal coating condition was for 1 h at 37 ℃,the dilution rate of serum sample was 1∶100,the optimal working concentration of the HRP-labeled rabbit anti-bovine IgG was 1∶1 000,sera and rabbit anti-bovine IgG should be incubated for 1 h at 37 ℃,the optimal confining liquid was 30 g/L skimmed milk in PBST,substrate should be incubated for 25 min at room temperature,and the threshold of the negative to the positive was 0.032.The 100 bovine serum samples collected from Yanbian Prefecture of Jilin Province were detected by the indirect ELISA,and the positive rate of the samples was 11.0%.The specificity experiment,reproducibility experiment and comparison experiment with agar diffusion test indicated that the indirect ELISA possessed satisfactory specificity,sensibility and reproducibility." @default.
- W2368500072 created "2016-06-24" @default.
- W2368500072 creator A5031938323 @default.
- W2368500072 date "2008-01-01" @default.
- W2368500072 modified "2023-09-27" @default.
- W2368500072 title "Development of an indirect ELISA for diagnosis of bovine enterotoxemia resulted from Clostridium perfringens type D" @default.
- W2368500072 hasPublicationYear "2008" @default.
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