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- W2368506569 abstract "Objective:To construct a small interfering RNA(siRNA)expression vector(psiRNA-VEGFR-3)targeting vascular endothelial growth factor receptor 3(VEGFR-3)and to investigate the effects of VEGFR-3 siRNA on the adherence and invasion of human colon cancer cells.Methods:A siRNA expression vector(psiRNA-VEGFR-3)targeting VEGFR-3 were constructed and was used to transfect LoVo cells via lipofectamine 2000.The mRNA and protein expression of VEGFR-3 were examined after transfection by reverse transcriptase polymerase chain reaction(RT-PCR)and Western blotting,respectively.The tumor adhesion ability was detected by cell-matrix adhesion experiment and the invasion ability of tumor cells was evaluated by millicell chamber model.Results:The VEGFR-3 siRNA expression vector was successfully constructed.The expression of VEGFR-3 mRNA and protein was inhibited after psiRNA-VEGFR-3 transfection.Seventy-two hours after psiRNA-VEGFR-3 transfection,Western blotting assay showed that the expression of VEGFR-3 protein was decreased from(1.26±0.19)to(0.39 s0.12)(P0.05),the adhesion ability of LoVo cells was also significantly decreased compared with the untransfected group and negative control group(0.407±0.029 vs 0.626±0.047,0.621±0.068,P0.01).The invasion assay demonstrated that the number of LoVo ceils penetrating the membrane in the transfection group was significantly lower than those in the untransfected and negative control group(6.38±3.25 vs 24.82±3.44,23.58±3.73,P0.05).Conclusion:The siRNA of VEGFR-3 gene can effectively inhibit the mRNA and protein expression of VEGFR-3 in LoVo cells,therefore restraining the adhesion and invasion ability of LoVo cells." @default.
- W2368506569 created "2016-06-24" @default.
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- W2368506569 date "2008-01-01" @default.
- W2368506569 modified "2023-09-25" @default.
- W2368506569 title "Inhibitory effect of VEGFR-3-targeted siRNA against adherence and invasion of human colon cancer cells" @default.
- W2368506569 hasPublicationYear "2008" @default.
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