Matches in SemOpenAlex for { <https://semopenalex.org/work/W2368518636> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2368518636 abstract "The study on extraction of DNA with improved CTAB(cetyl-trimethyl-ammonium bromide) method from Dendrocalamus hamiltonii Nees et Arn.ex Munro.It showed that CTAB buffer supplemented with 2%PVP(poly vinyl pyrrolidone) and 2%β-Mercaptoethanal was suitable for the genomic DNA extraction of D.hamiltonii Nees et Arn.ex Munro,with OD 260 /OD280 value of 1.7-1.9,representing high purity of the extracted DNA and the complete elimination of protein,phenol,pigment and RNA.Thus the improved CTAB method was approved suitable for further AFLP analysis of D.hamiltonii.Some key factors affecting DNA extraction,restriction-ligase reaction,preamplification and amplification processes were studied and an optimized AFLP reaction system of D.hamiltonii was established.With this AFLP reaction system,clear DNA electrophoresis fingerprints of D.hamiltonii were obtained,and the reproducibility was high.Eight primer combinations were filtrated from sixty and four pair with Mse I and Pst I,8 primer combinations with stable amplification and rich polymorphism for AFLP were screened,256 DNA bands were amplified by eight pairs of AFLP primers and 40.90%polymorphic bands per pair of primer were produced on average,which had 32 490 distinct and strong amplification bands with a bright background and 13 289 of them were polymorphic bands from 30 individuals of D.hamiltonii,we can entirely distinguish 30 individuals by 8primer combinations,which indicates that A FLP markers are useful for genetic analysis of D.hamiltonii" @default.
- W2368518636 created "2016-06-24" @default.
- W2368518636 creator A5031625902 @default.
- W2368518636 date "2010-01-01" @default.
- W2368518636 modified "2023-09-25" @default.
- W2368518636 title "Study on Genomic DNA Extraction Method and AFLP Amplification Reaction System of D.hamiltonii Nees et Arn.ex Munro" @default.
- W2368518636 hasPublicationYear "2010" @default.
- W2368518636 type Work @default.
- W2368518636 sameAs 2368518636 @default.
- W2368518636 citedByCount "0" @default.
- W2368518636 crossrefType "journal-article" @default.
- W2368518636 hasAuthorship W2368518636A5031625902 @default.
- W2368518636 hasConcept C104317684 @default.
- W2368518636 hasConcept C144024400 @default.
- W2368518636 hasConcept C149923435 @default.
- W2368518636 hasConcept C153523973 @default.
- W2368518636 hasConcept C153911025 @default.
- W2368518636 hasConcept C17757408 @default.
- W2368518636 hasConcept C178790620 @default.
- W2368518636 hasConcept C185592680 @default.
- W2368518636 hasConcept C2777563447 @default.
- W2368518636 hasConcept C2908647359 @default.
- W2368518636 hasConcept C49105822 @default.
- W2368518636 hasConcept C54355233 @default.
- W2368518636 hasConcept C552990157 @default.
- W2368518636 hasConcept C78063203 @default.
- W2368518636 hasConcept C81977670 @default.
- W2368518636 hasConcept C86803240 @default.
- W2368518636 hasConceptScore W2368518636C104317684 @default.
- W2368518636 hasConceptScore W2368518636C144024400 @default.
- W2368518636 hasConceptScore W2368518636C149923435 @default.
- W2368518636 hasConceptScore W2368518636C153523973 @default.
- W2368518636 hasConceptScore W2368518636C153911025 @default.
- W2368518636 hasConceptScore W2368518636C17757408 @default.
- W2368518636 hasConceptScore W2368518636C178790620 @default.
- W2368518636 hasConceptScore W2368518636C185592680 @default.
- W2368518636 hasConceptScore W2368518636C2777563447 @default.
- W2368518636 hasConceptScore W2368518636C2908647359 @default.
- W2368518636 hasConceptScore W2368518636C49105822 @default.
- W2368518636 hasConceptScore W2368518636C54355233 @default.
- W2368518636 hasConceptScore W2368518636C552990157 @default.
- W2368518636 hasConceptScore W2368518636C78063203 @default.
- W2368518636 hasConceptScore W2368518636C81977670 @default.
- W2368518636 hasConceptScore W2368518636C86803240 @default.
- W2368518636 hasLocation W23685186361 @default.
- W2368518636 hasOpenAccess W2368518636 @default.
- W2368518636 hasPrimaryLocation W23685186361 @default.
- W2368518636 hasRelatedWork W173152867 @default.
- W2368518636 hasRelatedWork W1972263550 @default.
- W2368518636 hasRelatedWork W2101025967 @default.
- W2368518636 hasRelatedWork W2106098300 @default.
- W2368518636 hasRelatedWork W2324965736 @default.
- W2368518636 hasRelatedWork W2355544110 @default.
- W2368518636 hasRelatedWork W2370063178 @default.
- W2368518636 hasRelatedWork W2590191276 @default.
- W2368518636 hasRelatedWork W2811242040 @default.
- W2368518636 hasRelatedWork W2924492604 @default.
- W2368518636 hasRelatedWork W3095863421 @default.
- W2368518636 hasRelatedWork W3144433127 @default.
- W2368518636 hasRelatedWork W3144514094 @default.
- W2368518636 hasRelatedWork W3147571025 @default.
- W2368518636 hasRelatedWork W3148408339 @default.
- W2368518636 hasRelatedWork W2243828124 @default.
- W2368518636 hasRelatedWork W2288575675 @default.
- W2368518636 hasRelatedWork W2317573813 @default.
- W2368518636 hasRelatedWork W2338251462 @default.
- W2368518636 hasRelatedWork W2593172046 @default.
- W2368518636 isParatext "false" @default.
- W2368518636 isRetracted "false" @default.
- W2368518636 magId "2368518636" @default.
- W2368518636 workType "article" @default.