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- W2368521308 abstract "Objective To explore the biological activity of the novel gene in tumor of hematology system,the expression of a novel human β3-glycosyltransferase(β3GnT8/β3GalT7) in 13 human haematopoietic cell lines was examined and the recombinant β3GnT8/β3GalT7 protein was obtained.Method mRNA expression of β3GnT8/β3GalT7 was semi-quantified by RT-PCR and normalized to the expression of the housekeeping gene β2-MG.The full length of β3GnT8/β3GalT7 cDNA except the predicted signal peptide was cloned by RT-PCR and the recombinant prokaryotic expression vector was constructed.After sequencing,the recombinant vector was transformed into E.coli M15 and His-tagged β3GnT8/β3GalT7 was induced by IPTG.The target protein was purified by Ni-NTA affinity chromatography column and characterized by SDS-PAGE and Western blot.Result β3GnT8/β3GalT7 was highly expressed in K562,U937,THP-1 and NB4 cell line,among which THP-1 reached the highest level.The prokaryotic expression vector pQE30-β3GnT8/β3GalT7 was successfully constructed and the purified target protein was obtained.Conclusion The human β3GnT8/β3GalT7 gene is expressed extensively in human haematopoietic cell lines with different expression levels.The recombinant pQE30-β3GnT8/β3GalT7 protein can be highly expressed in E.coli,which provides a basis for the further study on the biologic characteristic and mechanisms of β3GnT8/β3GalT7." @default.
- W2368521308 created "2016-06-24" @default.
- W2368521308 creator A5061480695 @default.
- W2368521308 date "2011-01-01" @default.
- W2368521308 modified "2023-09-26" @default.
- W2368521308 title "Expression of Human β3-Glycosyltransferase β3GnT8/β3GalT7 in Human Haematopoietic Cell Lines and Prokaryotic Expression and Characterization of the Gene" @default.
- W2368521308 hasPublicationYear "2011" @default.
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