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- W2368525909 abstract "Objective To determine brain dialysate and serum melatonin(MLT)levels in adult rats by high-performance liquid chromatography(HPLC)with fluorescence detection and enzyme-linked immunosorbent assay(ELISA),and compare the sensitivity,accuracy and reliability of the two detection methods. Methods The guide tube was implanted into the striatum of healthy adult rats. One week after implantation,dialysate samples were collected at 2:00,6:00,10:00,14:00,18:00,22:00hin striatum by brain microdialysis. Blood samples were collected from the rat tail vein at 22:00hon the following day. HPLC with fluorescence detection and ELISA were applied to detect dialysate and serum MLT levels. Results The lowest concentration of MLT that can be detected by HPLC with fluorescence detection was 0.1pg/mL. There was a regressive equation y=10 446 x+8 603.5(r=0.998 7,P0.05)in linear range 1.0~500pg/mL of MLT levels,with an intra-assay and inter-assay of below 6%. The detection limit of MLT by ELISA was 5.92pg/mL,and the detection range was 12.35~1 000pg/mL.The mean concentrations of dialysate MLT that showed a distinct fluctuation were(in pg/mL)(32.99±2.22),(18.25±2.27),(4.03±1.70),(2.92±1.08),(12.08±1.77),(21.83±2.04)at 2:00,6:00,10:00,14:00,18:00and 22:00hby HPLC with fluorescence detection. The recovery of the probes was from 12%to 13%. Besides,the mean concentration of serum MLT was(82.62±8.09)pg/mL by this method. The extraction recovery of serum MLT was more than 90%. There was a distinct positive correlation between serum MLT and dialysate MLT levels at 22:00pm,and pearson's correlation coefficient between the two was 0.987 at the 0.001level(two-tailed).In addition,the mean concentration of serum MLT was(81.31±9.62)pg/mL by ELISA. There was no significant difference in serum MLT levels between the two methods(P=0.115). Conclusion These data suggest that HPLC with fluorescence detection may be sensitive,rapid,accurate and reproducible in determination for MLT levels in dialysate and serum samples,and serum MLT levels by HPLC with fluorescence detection may be consistent with those by ELISA." @default.
- W2368525909 created "2016-06-24" @default.
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- W2368525909 date "2015-01-01" @default.
- W2368525909 modified "2023-09-24" @default.
- W2368525909 title "Determination of Brain Dialysate and Serum Melatonin Levels in Adult Rats" @default.
- W2368525909 hasPublicationYear "2015" @default.
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