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- W2368528096 abstract "Artificial nuclear transfer is a type of micromanipulator-independent nuclear transfer method. Oocyte denucleation and fusion of enucleated oocyte and donor somatic cell are the technological difficulty of artificial nuclear transfer. In this study,the artificial denucleation method of goat oocyte was optimized and the orbicular rate and enucleated rate of enucleated oocyte were 83. 45% ± 3. 2% and 98. 61% ± 1. 9%,respectively. The enucleated oocyte and donor somatic cell was fused by chemical assistant fusion method,and the fusion rate was 89. 05% ± 4. 33%. Cloned embryos were reconstructed using the optimized method above,and the cleavage rate and blastocyst rate were 83. 45% ± 2. 47% and 23. 4% ±5. 83%,respectively. In conclusion,the denucleation method of oocyte was optimized and the chemical assistant fusion method was constructed in this study,thus the goat somatic cell cloning technique system was successfully established on the basis of handmade nuclear transfer." @default.
- W2368528096 created "2016-06-24" @default.
- W2368528096 creator A5065680730 @default.
- W2368528096 date "2013-01-01" @default.
- W2368528096 modified "2023-09-25" @default.
- W2368528096 title "Artificial Somatic Cell Nuclear Transfer of Shaanbei White Cashmere Goat" @default.
- W2368528096 hasPublicationYear "2013" @default.
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