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- W2368557176 abstract "To study the effect of 60 Co γ radiation on gene expression and secretion of adrenomedullin and endothelin in the cultured rat vascular smooth muscle cells(VSMC). Rat VSMC were cultured in DMEM containing 10% FBS and were radiated by 60 Co γ at a doses of 1,14,25Gy respectively. Then mRNA level of adrenomedullin and endothelin in the VSMC were studied by reverse transcriptative competitive PCR. And the secretion of adrenomedullin and endothelin in the VSMC were studied by RIA. The secretion of Adm was increased by 270%( P 0.05) and 233%( P 0.05) with 14, 25Gy radiation respectively. The Adm mRNA levels were increased by 82.4%( P 0.01), 101%( P 0.01) respectively. The secretion of ET was decreased by 27.3%( P 0.01)and 58.0%( P 0.01) in VSMC with 14,25Gy radiation respectively. The ET mRNA levels were decreased by 47.1%( P 0.01)and 40.2%( P 0.01)in VSMC with 14,25Gy radiation respectively. In conclusion, 60 Co γ radiation can increase adm and decrease ET both at gene transcription and protein level." @default.
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- W2368557176 date "2002-01-01" @default.
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- W2368557176 title "Effects of γ radiation on the synthesis of adrenomedullin and endothelin in vascular smooth muscle cells of rat" @default.
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